We have studied general growth conditions of Gardnerella vaginalis, which is the predominant vaginal microorganism in women with bacterial vaginosis, and carried out biotyping of 15 wild strains isolated from bacterial vaginosis patients.
Standard
strain (ACTT 14018) of G. vaginalis had hemolytic activity to human blood, horse blood and sheep blood, but there were no hemolysis on geese, duck, beef and rabbit blood agar.
We have confirmed that transparent solid media adequate for good growth of G. vaginalis was Casman's agar supplemented with 5% Fildes enrichment and 5% horse serum. There was no visible colonial growth on Casman's agar patched with X(Haemin)and
V(nicotinamide adenine dinucleotide)factor strips.
It means that G. vaginalis needs another unknown growth factor besides X and V factors G. vaginalis have exhibited best growth in Casman's broth supplemented with 10% freezing thawing lysed human blood after 48 hours incubation, when growth was
measured by optical density.
In growth curve checked by the hour(Fig. 1), lag phase was about 6 hours after inoculation and growth was attained to stationary phase about 20 hours after inoculation.
In biotyping, we have identified biotpye 1,2,3,4,5,7 among eight kinds of biotypes, biotype 6, 8 were not typed, and type 7 was most common biotype(5/15 strains).
Finally we have observed that this bacteriae had survived for 28 days more at 4℃ anaerobic storage condition.
It that this short period storage condition is more suitable for short survival of bacteriae than another three conditions(Table 5).
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